Made In the San Francisco Bay Area
GigaYeast, Inc.

Kit Instructions

Step 1:  Carefully Open the Box

Open the box using a knife to carefully slice the sealing tape without damaging the box itself. Do not throw away the box!  You are going to ship the samples in it…

Carefully open the box using a sharp knife to slice the sealing tape

Carefully open the box using a sharp knife to slice the sealing tape

Step 2:  Label all collection containers

Clearly label each tube with a sample name (i.e. Fermenter 1, Bright tank 6, lot#00743 etc.). Use a sharpie or other permanent marker.
label

Step 3:  Wash hands and put on clean latex gloves

Wash hands and forearms thoroughly in warm water and soap all the way up to the elbows. Dry off with a clean towel and put on latex gloves. You can spray alcohol or other sanitizer on the gloves

Washing hands and forearms is one of the most important steps for sanitary sampling

Washing hands and forearms is one of the most important steps for sanitary sampling

 

Step 4:  Take a Sample form a Fermenter or Bright Tank

 

Start by sanitizing the valve: First make sure the valve is clean and free of debris. Then see below depending on valve type.

For a dedicated sampling valve:  Fill valve with 70% alcohol and briefly flame to burn off the alcohol.

Use tubing to fill the valve completely with 70% alcohol

Use tubing to fill the valve completely with 70% alcohol

Flame the valve long enough to burn off the alcohol

Flame the valve long enough to burn off the alcohol

For a standard valve:  Attach a 90 degree elbow to the valve so that it can be filled with sanitizer (i.e. PAA or similar). Let the valve soak for 5-10’ and then remove the elbow and attach a sanitized barb for sample collection.

Attach a clean 90 degree elbow to the valve

Attach a clean 90 degree elbow to the valve

Fill the elbow with sanitizer and let stand for 5' to 10'

Fill the elbow with sanitizer and let stand for 5′ to 10′

 

Collect the sample:  Run off liquid for 5 seconds to flush sanitizer. Collect 20-30 ml into sterile tube provided by holding the tube 0.5 to 1” below the barb or valve without touching the tube or anything else. Firmly tighten the cap after filling.

Run off liquid to flush sanitizer.

Run off liquid to flush sanitizer.

carefully transfer liquid to the sample container without touching the valve/barb to anything.

carefully transfer liquid to the sample container without touching the valve/barb to anything.

 

Sample packaged beer:  Open bottle/can in a clean, dry location. Flame the lip of the container thoroughly. Pour off approximately ½ the beer to a waste container. Gently swirl the remaining beer to resuspend any settled yeast/microbes. Carefully pour 20-30 ml into a sample tube. Firmly tighten the cap after filling.

thoroughly flame the lip of the container

thoroughly flame the lip of the container

Pour off 1/2 the beer and gently swirl to resuspend the remainder

Pour off 1/2 the beer and gently swirl to resuspend the remainder

Carefully pour the beer into a sterile container--  without touching the lip to the container or anything else.

Carefully pour the beer into a sterile container– without touching the lip to the container or anything else.

 

Sample a surface:  Carefully remove the sterile swab from its tube by touching only the end of the wooden stick. Firmly swab over an area of 4 square inches.   Drop the swab back in the sterile tube cotton side down without touching the swab tip or the lip of the tube. Firmly tighten the cap.

hold the swab as far away from the cotton tip as possible while swabbing a surface

hold the swab as far away from the cotton tip as possible while swabbing a surface

Drop the swab into a sterile container holding it from as far away from the cotton tip as possible

Drop the swab into a sterile container holding it from as far away from the cotton tip as possible

 

Step 5: Seal Tube With Parafilm

Seal each filled tube with a strip of Parafilm (included). First make sure the cap is tight. Then remove the paper backing from Parafilm and stretch the film to wrap 2-3 times around area where the cap meets the tube. This is important to ensure a leak proof seal during shipping. Return each tube to its own bag.

Stretch the parafilm to wrap around the area where the cap meets the tube

Remove the paper backing

Gently stretch the parafilm to wrap 2-3 x around the area where the cap meets the tube

Gently stretch the parafilm to wrap 2-3 x around the area where the cap meets the tube

 

Step 6: Package and Ship

Seal each tube in its own bag and return to the box they were shipped in. Make sure all tubes are labeled and bags/tubes are sealed properly. Seal the box with packing tape, place return shipping label over original shipping label and deliver to any fed ex driver or location.

Make sure all the tubes are sealed and placed in a bag

Make sure all the tubes are sealed and placed in a bag

Seal the box with packing tape

Seal the box with packing tape

place the return shipping label over the original label

place the return shipping label over the original label

Step 7: RESULTS

Your results will be sent to the email address provided within 7 days of shipping. Contact us at Laboratory@GigaYeast.Com if you have any questions.